Mixing and Reconstitution of Neurotoxins

The important factors of mixing and reconstitution on neurotoxins such as Botox, Dysport, and Xeomin are:

• Sterility: The reconstitution solution and the neurotoxin vial must be sterile. This is to prevent the introduction of bacteria or other microorganisms that could cause infection.
• Temperature: The reconstitution solution and the neurotoxin vial must be at room temperature (77°F to 86°F) before mixing. Mixing at colder temperatures can cause the neurotoxin to precipitate out of solution.
• Agitation: The neurotoxin must be agitated gently during mixing. This helps to ensure that the reconstituted solution is homogeneous. Excessive agitation can cause foaming, which can lead to loss of product.
• Time: The reconstituted solution must be used within 24 hours of mixing. After 24 hours, the neurotoxin may lose its potency.

In addition to these factors, it is also important to follow the specific instructions that come with the neurotoxin product. These instructions may vary depending on the brand of neurotoxin and the specific formulation.

Here is a summary of the important factors of mixing and reconstitution on neurotoxins:

• Sterility: The reconstitution solution and the neurotoxin vial must be sterile.
• Temperature: The reconstitution solution and the neurotoxin vial must be at room temperature before mixing.
• Agitation: The neurotoxin must be agitated gently during mixing.
• Time: The reconstituted solution must be used within 24 hours of mixing.
• Instructions: It is important to follow the specific instructions that come with the neurotoxin product.

The differences of mixing and reconstitution on neurotoxins such as Botox, Dysport, and Xeomin are:

• Reconstitution solution: Botox, Dysport and Xeomin are reconstituted with saline solution.
• Volume of reconstitution solution: Botox is reconstituted with 0.9 mL of saline solution, while Dysport is reconstituted with 2.5 mL of bac water and Xeomin is reconstituted with 0.5 mL of saline solution.
• Number of units per 0.1 mL: Botox contains 10 units per 0.1 mL, Dysport contains 30 units per 0.1 mL, and Xeomin contains 5 units per 0.1 mL.
• Onset of action: Botox and Xeomin have an onset of action of 2-3 days, while Dysport has an onset of action of 1-2 days.
• Duration of action: Botox and Xeomin generally have an onset of action of 3-7 days, not 2-3 days. Dysport may have a slightly faster onset of 2-3 days. The duration of action for Botox and Xeomin is usually 3-4 months, while Dysport can last 3-4 months, not 4-6 months as stated[3][5].

Here is a table summarizing the differences of mixing and reconstitution on neurotoxins:

It is important to note that these are just general guidelines. The specific instructions for mixing and reconstituting neurotoxins may vary depending on the brand of neurotoxin and the specific formulation. It is always important to follow the instructions that come with the product.

The information provided about the mixing and reconstitution of neurotoxins such as Botox, Dysport, and Xeomin is mostly accurate but requires some clarifications and updates based on the latest guidelines and research.

In summary, while the general principles of sterility, gentle agitation, and timely use are accurate, specific details such as reconstitution solutions and volumes should be verified against the latest manufacturer guidelines and clinical practices.

Citations:
[1] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3935649/
[2] https://www.asds.net/Portals/0/PDF/asdsa/white-paper-storage-and-reuse-of-reconstituted-neuromodulators.pdf
[3] https://www.mdpi.com/2072-6651/10/12/535
[4] https://www.accessdata.fda.gov/drugsatfda_docs/label/2023/125274s125lbl.pdf
[5] https://www.dysport.com/en-us/hcp/dilution-and-reconstitution
[6] https://www.aetna.com/cpb/medical/data/100_199/0113.html
[7] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695379/